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Effect on splenocyte proliferation in weaned piglets induced by lipopolysaccharide (LPS) challenge. Data represent the mean ± standard deviation (n = 6 in each group) ** – highly significant difference (P-value < 0.01)

Journal: Journal of Veterinary Research

Article Title: Effect on the splenocyte function of weaned piglets induced by continuous lipopolysaccharide injections

doi: 10.2478/jvetres-2024-0024

Figure Lengend Snippet: Effect on splenocyte proliferation in weaned piglets induced by lipopolysaccharide (LPS) challenge. Data represent the mean ± standard deviation (n = 6 in each group) ** – highly significant difference (P-value < 0.01)

Article Snippet: Next, the splenocyte suspension was adjusted to a concentration of 5 × 10 6 cells/mL and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (11875; Solarbio) supplemented with 10% foetal bovine serum (13011-8611; Tianhang Biotechnology, Huzhou, China) and 1% penicillin-streptomycin (P1400; Solarbio) in a humidified incubator at 37°C with 5% CO 2 .

Techniques: Standard Deviation

Flow cytometry results of evaluation of weaned piglet splenocyte cell cycle distribution after lipopolysaccharide (LPS) challenge. A, B, C and D show the splenocytes of saline-treated piglets on day (d)1, d5, d9 and d13, respectively; a, b, c and d show the splenocytes of LPS-treated piglets on the same days; G0 – cells in resting phase; G1 – cells in gap 1 phase; S – cells in synthesis phase; G2 – cells in gap 2 phase; M – cells in mitosis phase; FL2-A – total relative fluorescent intensity in channel 2; PI-A – total fluorescent intensity from propidium iodide

Journal: Journal of Veterinary Research

Article Title: Effect on the splenocyte function of weaned piglets induced by continuous lipopolysaccharide injections

doi: 10.2478/jvetres-2024-0024

Figure Lengend Snippet: Flow cytometry results of evaluation of weaned piglet splenocyte cell cycle distribution after lipopolysaccharide (LPS) challenge. A, B, C and D show the splenocytes of saline-treated piglets on day (d)1, d5, d9 and d13, respectively; a, b, c and d show the splenocytes of LPS-treated piglets on the same days; G0 – cells in resting phase; G1 – cells in gap 1 phase; S – cells in synthesis phase; G2 – cells in gap 2 phase; M – cells in mitosis phase; FL2-A – total relative fluorescent intensity in channel 2; PI-A – total fluorescent intensity from propidium iodide

Article Snippet: Next, the splenocyte suspension was adjusted to a concentration of 5 × 10 6 cells/mL and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (11875; Solarbio) supplemented with 10% foetal bovine serum (13011-8611; Tianhang Biotechnology, Huzhou, China) and 1% penicillin-streptomycin (P1400; Solarbio) in a humidified incubator at 37°C with 5% CO 2 .

Techniques: Flow Cytometry, Saline